DNA

Approximately 3 mg/ml Protein AG is immobilized on non-cross-linked beaded magnetic agarose where iron is encapsulated by agarose. The beads have excellent magnetic properties allowing rapid and efficient capture and low non-specific binding using a Magnetic Bead Stand*. These beads are stable at pH 2-11. The product is supplied as 50% suspension of Protein AG magnetic agarose in 20% ethanol aqueous suspension when packed. *NextGen Bioproducts also sells Magnetic Bead Stand TECHNICAL SPECIFICATIONS BEAD GEOMETRY & SIZE:    Spherical, ∼ 5 – 10 μm diameter (Encapsulated with Iron) CROSSLINKED:                       NO ACTIVATING GROUP:            Carbonyl MATRIX STABILITY:               Stable in all commonly used reagents STORAGE SOLUTION:            20% ethanol aqueous STORAGE TEMPERATURE:    4°C to 8°C.   DO NOT FREEZE. STABILITY:                              Stable for 1 year under proper storage condition

Protein G PLUS Agarose resin consists of Recombinant Protein G (≥ 5 mg Protein G / ml resin) covalently coupled to cross-linked activated agarose beads. It provides a very stable bond that can greatly minimize leakage of the Protein G allowing for reuse of the affinity resin in several purification steps. The resin works well in batch or column purifications. The product is also suitable for immunoprecipitation (IP) procedures. This product is supplied as 50% suspension of Protein G Agarose Resin in 20% ethanol. The settled bead volume is as per each product pack. TECHNICAL SPECIFICATIONS BEAD GEOMETRY & SIZE:  Spherical, ∼ 50 – 150 μm diameter CROSSLINKED:                       Yes BEAD CROSSLINKING %:     6% ACTIVATING GROUP:           Carbonyl MATRIX STABILITY:             Stable in all commonly used reagents STORAGE SOLUTION:           20% aqueous ethanol STORAGE TEMPERATURE: 4°C to 8°C.   DO NOT FREEZE. STABILITY:                             Stable for 1 year under proper storage condition

Recombinant Streptavidin purified from E.coli is covalently bound to 6% cross-linked agarose bead. The Streptavidin-linked resin which is stable at pH 2-11 can be used to separate biotinylated products from non-biotinylated products. Biotinylated molecules can be RNA, DNA or protein which binds to the Streptavidin agarose bead with extremely high affinity. This high-affinity interaction is useful to monitor protein-RNA, protein-DNA, and protein-protein interaction. Product Description: Approximately 1.5 mg/ml Streptavidin has been immobilized on 6% cross-linked beaded agarose. This product is supplied as 50% suspension of Streptavidin Resin in aqueous ethanol when packed. TECHNICAL SPECIFICATIONS BEAD GEOMETRY & SIZE:  Spherical, ∼ 50 – 150 μm diameter CROSSLINKED:                       Yes BEAD CROSSLINKING %:     6% ACTIVATING GROUP:           Carbonyl MATRIX STABILITY:             Stable in all commonly used reagents STORAGE SOLUTION:           20% aqueous ethanol STORAGE TEMPERATURE: 4°C to 8°C.   DO NOT FREEZE STABILITY:                             Stable for 1 year under proper storage condition  

Murine Leukemia Virus Reverse Transcriptase (MuLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5 Kbp). The enzyme is isolated from E.coli expressing the modified pol gene of MuLV on a plasmid. This modified version of RT is thermotolerant (working temp 42 – 52⁰C using ng level of total RNA). The RNase H activity of MuLV RT is weaker than the commonly used AMV- Reverse Transcriptase. Reagents supplied • Super RT (200 U/µl) , 100 µl • 5x RT Buffer (250 mM Tris-HCl, pH 8.4; 375 mM KCl; 15 mM MgCl2), 400 µl • dNTP mix (10mM), 50 µl • Oligo (dT) mix (500 µg/ml), 50 µl • Random Hexamer (50 ng/ul), 50 ul • 0.1 M DTT, 100 µl • Murine RNase Inhibitor (1 U/µl), 50 µl • DEPC H2O, 600 µl Storage Buffer: 20mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 50% glycerol (v/v). Storage Instruction: -20°C

Taq DNA Ploymerase is cloned version of Taq DNA Polymerase which is expressed in E. coli. This recombinant Taq DNA Polymerase shows identical characteristics to native Taq from Thermus aquaticus with respect to activity, specificity, thermostability and performance in PCR. Taq DNA Polymerase catalyzes the incorporation of dNTPs into DNA. It requires a DNA template, a primer terminus, and the divalent cation Mg++. Taq DNA Polymerase contains a polymerization dependent 5′-3′ exonuclease activity. It does not have a 3′-5′ exonuclease and thus no proof-reading function. Despite this, the enzyme synthesizes DNA in vitro with reasonable fidelity. Reagents supplied: Taq DNA Polymerase, (2.5 U/µl) 10X Taq Reaction buffer (100 mM Tris-HCl, pH 8.8; 500 mM KCl; 0.1% Tween 20; 15 mM MgCl2; 0.1% Tween 20) Storage Buffer:50 mM Sodium Phosphate, pH 8; 300 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 50% glycerol(v/v) Storage instructions: -20°C