DNA

Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endo-toxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. The human IL-1 beta cDNA encodes a 269 amino acid precursor. A 116 amino acid pro-peptide is cleaved intra-cellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine. The 17 KDa mature human IL-1 beta shares >80 % amino acid sequence identity with mouse IL-1 beta.
Protein Sequence: APVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLFESAQFPNWYISTSQAENM PVFLGGTKGGQDITDFTMQFVSS

Purity: >98%, by SDS-PAGE under reducing conditions and visualized by Coomassie stain.
Formulation: Supplied as a 0.2 μm filtered solution in PBS at 50 μg(1mg/ml) in sterile PBS containing 50% Glycerol.

Storage buffer: Phosphate Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions:   -20°C (Recommended).

Tumor necrosis factor alpha (TNF-α), is a member of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation and immune system development. TNF-α is produced by a wide variety of cell types including lymphoid cells. TNFa matures through separation of the extracellular domain from rest of the molecule. It forms a soluble trimer which binds the trimeric TNF receptor (TNFR). TNF-α is a key cytokine in the development of several inflammatory disorders.
Protein Sequence:
MSTESMIRDVELAEEALPKKTGGPQGSRRCLFLSLFSFLIVAGATTLFCLLHFGVIGPQREESPRDLSLISPLAQAVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL

Purity: > 98%, by SDS-PAGE under reducing conditions and visualized by Coomassie stain.
Formulation: Supplied as a 0.2 μm filtered solution in PBS at 100 μg (1mg/ml) in sterile PBS containing at least 0.1% human or bovine serum albumin.

Storage buffer: Phosphate Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions: -20°C (Recommended).

NB-Mini Plasmid Isolation Kit is a spin column-based kit which is based on silica membrane purification technology.

The silica membrane in presence of chaotropic agent under low pH efficiently adsorbs DNA which can be easily eluted in neutral pH (~8.0).

This kit can efficiently extract and purify plasmid DNA from bacteria. The purified plasmid DNA is suitable for basic molecular biology techniques like restriction digestion, PCR amplification, sequencing etc.

Kit Contents:                 # BB-PIK50 | 250 (50/250 preps)
Suspension Buffer:         15 ml
Lysis Buffer:                   15 ml
Neutralization Buffer:     20 ml
Wash Buffer 1:                18 ml
Wash Buffer 2:                15 ml
Elution Buffer:                 15 ml
RNase A:                          150 µl
Spin Column:                   50 nos
Collection tubes:              50 nos

Storage:
NB Mini Plasmid Isolation Kit (# BB-PIK50) is stable for 12 months if stored properly in dry place at room temperature (RT, 20°C – 25°C) not exposed to sunlight.

Nitrilotriacetic acid (NTA)-crosslinked-agarose resin consists of NTA groups covalently immobilized on agarose beads by stable ether linkages via a spacer arm. NTA is a tetradentate chelating agent forms octahedral coordination complex with divalent transition metal cations (Ni2+) along with two water molecules occupied in cis manner. This ready to use resin, after loading with a divalent transition metal ion (Ni2+) is ideal for rapid purifications of His-tagged proteins. In comparison with other chelating resins such as iminodiacetic acid (IDA)-agarose, the NTA has two sites (cis) available for the interaction with imidazole of His-tagged proteins, instead of the three in IDA, and so have lesser probability of nonspecific binding. NTA resins are usually more easily regenerated, allowing a better elution of the fused proteins bound with smaller concentrations of imidazole.
Product is supplied as 50% suspension of Ni-NTA Agarose resin in 20% aqueous ethanol; the settled bead volume was measured in ml when supplied.
Under optimum condition, 1.0 ml of Ni-NTA Agarose Bead can purify up to 50 mg of His-tagged protein.

Ni-NTA Agarose Bead can withstand autoclaving at 120°C for 20 min without any significant loss of binding efficiency.

TECHNICAL SPECIFICATIONS
BEAD GEOMETRY & SIZE:     Spherical, ∼ 50 – 150 μm diameter
CROSS-LINKED:                       Yes
BEAD AGAROSE %:                4%
ACTIVATING GROUP:            Epoxy
MATRIX STABILITY:               Stable in all commonly used reagents
STORAGE SOLUTION:           20% Ethanol
STORAGE TEMPERATURE:    4°C to 8°C.    DO NOT FREEZE

Maximum loading volume of this ready to load Pre-Stained Protein Ladder is 5 μl in a standard PAGE.

Pre-stained Protein Ladder is a mixture of seven highly purified recombinant proteins covalently coupled to a dye that resolves into 7 sharp bands when electrophoresed. The protein concentrations are carefully balanced for even intensity. The orange 35 KDa band serves as the reference indicator. The apparent molecular weights of the Pre-stained Protein Ladder bands were determined on Tris-glycine SDS-PAGE gels by comparison to the unstained Protein Ladder. This Pre-stained Protein Ladder is suitable to use in SDS-PAGE and western blotting applications. It allows continuous monitoring of protein separations during electrophoresis and also provides a quick and easy way to assess blotting efficiency.
The Pre-stained Protein marker comes ready for direct loading on a SDS-PAGE. No need to do any other processing for loading in a SDS PAGE. Mix well (vortex) and directly load on the gel.
The Pre-stained Protein marker should be aliquoted into multiple tubes to prevent repeated freeze & thaw.

Storage Condition: Store at –20°C.