DNA

PNK (polynucleotide kinase) is a DNA repair enzyme that participates in single strand break repair and non-homologous end rejoining (NHEJ) for double strand breaks. PNK possesses a 5’-DNA kinase activity and a 3’-DNA phosphatase activity. It has three domains, a C-terminal kinase domain, a central phosphatase domain, and an N-terminal forkhead associated (FHA) domain that is responsible for protein-protein interactions. Reduction in expression of PNK by RNAi sensitizes cells to ionizing radiation and topoisomerase I inhibitors. Purification: Purified against PNK protein affinity chromatography. Recommended Dilutions: Western Blotting 1:2000; ELISA 1:1000 Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide. Storage instructions: Store at -20°C (Recommended). *N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubations and washings are in 1X TBS, 0.1% Tween-20. Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.  

Nitrilotriacetic acid (NTA)-crosslinked-agarose resin consists of NTA groups covalently immobilized on agarose beads by stable ether linkages via a spacer arm. NTA is a tetradentate chelating agent forms an octahedral coordination complex with divalent transition metal cations (Co2+) along with two water molecules occupied in cis manner. This ready to use resin, after loading with a divalent transition metal ion (Co2+) is ideal for rapid purifications of His-tagged proteins. In comparison with other chelating resins such as iminodiacetic acid (IDA)-agarose, the NTA has two sites (cis) available for the interaction with imidazole of His-tagged proteins, instead of the three in IDA, and so have a lesser probability of nonspecific binding. NTA resins are usually more easily regenerated, allowing a better elution of the fused proteins bound with smaller concentrations of imidazole. Though Ni-resin is being widely used for His-tag recombinant protein purification but in some cases, Co-resins is preferable due to its higher specificity. Where purity is of prior importance over quantity, it is recommended to use Co-resins for better results. The product is supplied as 50% suspension of Co-NTA Agarose resin in 20% aqueous ethanol; the settled bead volume is measured in ml, when supplied. Co-NTA resins can withstand autoclaving at 120°C for 20 min without any significant loss of binding efficiency.  TECHNICAL SPECIFICATIONS BEAD GEOMETRY & SIZE:  Spherical, Standard ∼ 50-150 μm diameters CROSS-LINKED:                    Yes AGAROSE %:                          4% ACTIVATING GROUP:           Epoxy MATRIX STABILITY:             Stable in all commonly used reagents STORAGE SOLUTION:          20 % Ethanol STORAGE TEMPERATURE:  4°C to 8°C.  DO NOT FREEZE. For Research use only – Not for use in diagnostics procedures.

  This DNA ladder premixed with loading dye (Bromophenol blue and Xylene Cyanol) can be used for direct loading on gel (~5 µl/ lane). Concentration: 0.1μg/μl Storage: -20°C Note: 5 µl (i.e. 500 ng) loading is recommended.

  This DNA ladder premixed with loading dye (Bromophenol blue and Xylene Cyanol). 10 µl loading is recommended. Concentration: 0.05 µg/µl Storage: -20^OC

NB-Gel Extraction Kit is a column based nucleic acid purification kit where the column has silica membrane which is capable of purifying DNA fragments easily from TAE or TBE agarose gel (both normal and low melting point agarose) with significant removal of proteins and other organic impurities utilizing the optimized buffer system. This kit can purify DNA fragments of 80 bp to 10Kbp with a recovery rate of more than 75%. The purified DNA is suitable for basic molecular biology experiments like restriction digestion, ligation, PCR amplification etc. Kit Contents:            # BB-GEK50 | 250 (50/250 preps) Binding Buffer:            25 ml Wash Buffer:               15 ml Elution Buffer:            15 ml Spin Column:              50 nos Collection Tubes:       50 nos (2.0 ml) Storage: NB Gel Extraction Kit (# BB-GEK50) is stable for 12 months if stored properly in dry place at room temperature (20°C – 25°C) not exposed to sunlight.