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Anti GAPDH Antibody Original price was: $200.00.Current price is: $150.00.
GAPDH is an enzyme of ~37kDa that catalyzes the conversion of glyceraldehyde 3-phosphate in the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In addition to this long-established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, and ER to Golgi vesicle shuttling. Anti-GAPDH antibody is primarily used to test purity of cytoplasmic cell fractionation and loading control. This antibody is produced in rabbit by repeated injections of Immunogen. Purification: Purified against GAPDH protein (residues 13-335) affinity chromatography. Recommended Dilutions: Western Blotting-1:2000; ELISA-1:10,000 Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide. Storage instructions: Store at -20°C (Recommended). *N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubations and washings are in 1X TBS, 0.1% Tween-20. Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.  
Anti- FLAG Antibody Original price was: $200.00.Current price is: $150.00.
Description: Proteins of interest are generally tagged with FLAG peptide for easy checking of their expression in recombinant strains. Anti-FLAG antibody is the tool for detection of FLAG-tag proteins by different immunological techniques. This antibody is produced in rabbit by repeated injections of Immunogen. Purification: Purified against peptide affinity chromatography. Recommended Dilutions: Western Blotting-1:1000; ELISA-1:1000 Storage buffer: Tris Buffer pH 7.4, containing 1% BSA & 50% Glycerol without azide. Storage instructions: Store at -20°C (Recommended).
Anti- β Actin Antibody Original price was: $200.00.Current price is: $150.00.
Description: Beta actin is a ~42 KDa mammalian protein. Anti-β actin antibody is primarily used to test purity of cytoplasmic cell fractionation and loading control. This antibody is produced in rabbit by repeated injections of Immunogen. Purification: Purified against Beta actin peptide affinity chromatography. Recommended Dilutions: Western Blotting-1:2000-5000 Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide. Storage instructions: Store at -20°C (Recommended).
Anti- γ Tubulin Antibody Original price was: $200.00.Current price is: $150.00.
Description: Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. As a critical part of the microtubule-organizing center (MTOC), the third member of the tubulin superfamily, γ-tubulin, is required for microtubule nucleation as well as centrosome duplication and spindle assembly. Gamma tubulin is a ~49 KDa mammalian protein. Anti-γ tubulin antibody is primarily used to test purity of cytoplasmic cell fractionation and loading control. This antibody is produced in rabbit by repeated injections of Immunogen. Purification: Purified against gamma tubulin protein affinity chromatography. Recommended Dilutions: Western Blotting-1:2,000-5,000; ELISA-1:1,000 Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide. Storage instructions: Store at -20°C (Recommended).
Anti-Erk2/MAPK1 Antibody Original price was: $200.00.Current price is: $150.00.
Description: Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42MAPK (ERK1/2) signalling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines. Anti-Erk2 antibody can be used for detection of Erk2 protein by immunological techniques. This antibody is produced in rabbit by repeated injections of Immunogen. Purification: Purified against Erk2 protein affinity chromatography. Recommended Dilutions: Western Blotting-1:5,000; ELISA-1:1,000 Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide. Storage instructions: Store at -20°C (Recommended).
Anti-H3 Antibody Original price was: $200.00.Current price is: $150.00.
Description: Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. This antibody is produced in rabbit by repeated injections of Immunogen. Purification: Purified against peptide affinity chromatography. Recommended Dilutions: Western Blotting-1:10,000; ELISA-1:5,000 Storage buffer: Tris Buffer pH 7.4, containing 1% BSA and 50% Glycerol without azide. Storage instructions: Store at -20°C (Recommended).
Anti-His Antibody Original price was: $200.00.Current price is: $150.00.
Proteins of interest are generally tagged with His peptide for both an easy detection and simple purification by Ni2+-affinity chromatography. Anti-His antibody is the tool for detection of His-tag proteins by different immunological techniques. This antibody is produced in rabbit by repeated injections of Immunogen. Purification: Purified against peptide affinity chromatography. Recommended Dilutions: Western Blotting-1:2000; ELISA-1:10,000 Storage buffer: Tris Buffer pH 7.4, containing 1% BSA & 50% Glycerol without azide. Storage instructions: Store at -20°C (Recommended). *N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubations and washings are in 1 X TBS, 0.1% Tween-20. Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.

Anti-NEIL2 Antibody Original price was: $200.00.Current price is: $150.00.
Description: NEIL1, NEIL2 and NEIL3, also known as Endonuclease VIII-like 1, 2 and 3. NEIL 1, 2 and 3, are nuclear proteins involved in the repair of DNA damaged by oxidation. The NEIL proteins belong to the FPG family of proteins. They act as DNA glycosylases that can recognize and remove damaged bases, leaving an abasic site. Purification: Purified by antigen affinity chromatography using NEIL2 protein. Recommended Dilutions: Western Blotting- 1:1,000 Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide.
Anti-NEMO/IKKγ Antibody Original price was: $200.00.Current price is: $150.00.
Description: The cytosolic NFκB/Rel transcription factors are in inactive state, complexed with the inhibitory IκB proteins. Phosphorylation-induced, proteasome-mediated degradation of IκB triggers their activation. The key regulatory step in this pathway involves activation of IκB kinase (IKK) complex, whose catalysis is generally carried out by three tightly associated IKK subunits. IKKγ and IKKβ serve as the catalytic subunits of the kinase. IKK serves as the regulatory subunit. Phosphorylation of serines 177 and 181 in the activation loop of IKKβ (176 and 180 in IKKα) causes conformational changes resulting in activation of IKK. Anti- IKKγ antibody can be used for IKK2/β kinase assay from immunoprecipitated (IP) cell extracts. This antibody is produced in rabbit by repeated injections of Immunogen. Purification: Purified against IKKγ protein (residues 40-210) affinity chromatography. Recommended Dilutions: Western Blotting -1:2,000; ELISA-1:1,000; IP-1:100 Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide. Storage instructions: Store at -20°C (Recommended).
Anti-p50 Antibody Original price was: $200.00.Current price is: $150.00.
The transcription factors NF-κB/Rel family play a pivotal role in inflammatory and immune responses. There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins. NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression. NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus. Following IKK-mediated phosphorylation of p105 NF-κB at multiple sites (Ser921, 923, 927, and 932) on its carboxy-terminus, SCF/β-TrCP mediated processing produces the 50 kDa active form p50. Anti-P50 antibody can be used for detection of P50 protein by immunological techniques. This antibody is produced in rabbit by repeated injections of Immunogen. Purification: Purified against P50 (residues 1-430) protein affinity chromatography. Recommended Dilutions: Western Blotting-1:30,000; ELISA-1:10,000; ChIP/IP-1:1000 Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide. Storage instructions: Store at -20°C (Recommended). *N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubations and washings are in 1X TBS, 0.1% Tween-20. Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.
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