Ni-NTA Agarose Beads

Nitrilotriacetic acid (NTA)-crosslinked-agarose resin consists of NTA groups covalently immobilized on agarose beads by stable ether linkages via a spacer arm. NTA is a tetradentate chelating agent forms octahedral coordination complex with divalent transition metal cations (Ni2+) along with two water molecules occupied in cis manner. This ready to use resin, after loading with a divalent transition metal ion (Ni2+) is ideal for rapid purifications of His-tagged proteins. In comparison with other chelating resins such as iminodiacetic acid (IDA)-agarose, the NTA has two sites (cis) available for the interaction with imidazole of His-tagged proteins, instead of the three in IDA, and so have lesser probability of nonspecific binding. NTA resins are usually more easily regenerated, allowing a better elution of the fused proteins bound with smaller concentrations of imidazole.
Product is supplied as 50% suspension of Ni-NTA Agarose resin in 20% aqueous ethanol; the settled bead volume was measured in ml when supplied.
Under optimum condition, 1.0 ml of Ni-NTA Agarose Bead can purify up to 50 mg of His-tagged protein.

Ni-NTA Agarose Bead can withstand autoclaving at 120°C for 20 min without any significant loss of binding efficiency.

TECHNICAL SPECIFICATIONS
BEAD GEOMETRY & SIZE:     Spherical, ∼ 50 – 150 μm diameter
CROSS-LINKED:                       Yes
BEAD AGAROSE %:                4%
ACTIVATING GROUP:            Epoxy
MATRIX STABILITY:               Stable in all commonly used reagents
STORAGE SOLUTION:           20% Ethanol
STORAGE TEMPERATURE:    4°C to 8°C.    DO NOT FREEZE