Co-NTA Agarose Beads

Nitrilotriacetic acid (NTA)-crosslinked-agarose resin consists of NTA groups covalently immobilized on agarose beads by stable ether linkages via a spacer arm. NTA is a tetradentate chelating agent forms an octahedral coordination complex with divalent transition metal cations (Co2+) along with two water molecules occupied in cis manner. This ready to use resin, after loading with a divalent transition metal ion (Co2+) is ideal for rapid purifications of His-tagged proteins. In comparison with other chelating resins such as iminodiacetic acid (IDA)-agarose, the NTA has two sites (cis) available for the interaction with imidazole of His-tagged proteins, instead of the three in IDA, and so have a lesser probability of nonspecific binding. NTA resins are usually more easily regenerated, allowing a better elution of the fused proteins bound with smaller concentrations of imidazole.

Though Ni-resin is being widely used for His-tag recombinant protein purification but in some cases, Co-resins is preferable due to its higher specificity. Where purity is of prior importance over quantity, it is recommended to use Co-resins for better results. The product is supplied as 50% suspension of Co-NTA Agarose resin in 20% aqueous ethanol; the settled bead volume is measured in ml, when supplied.
Co-NTA resins can withstand autoclaving at 120°C for 20 min without any significant loss of binding efficiency.

 TECHNICAL SPECIFICATIONS
BEAD GEOMETRY & SIZE:  Spherical, Standard
∼ 50-150 μm diameters
CROSS-LINKED:                    Yes
AGAROSE %:                          4%
ACTIVATING GROUP:           Epoxy
MATRIX STABILITY:             Stable in all commonly used reagents
STORAGE SOLUTION:          20 % Ethanol
STORAGE TEMPERATURE:  4°C to 8°C.  DO NOT FREEZE.
For Research use only – Not for use in diagnostics procedures.