Glutathione Agarose Beads

This product consists of reduced glutathione attached to epoxy activated 4% cross-linked beaded agarose through the sulfur atom with a 12 atom (10 carbon) spacer arm. This 12 atom spacer arm between the bead and the ligand allows strong affinity interaction between glutathione & GST part of the fusion protein and minimizes steric inhibition.
Affinity chromatography using glutathione-agarose permits rapid, mild, non-denaturing, and highly selective purification of glutathione binding enzymes such as GST, glutathione peroxidase, and glyoxalase I. GST-tagged fusion proteins or other glutathione-dependent proteins can be purified directly from bacterial lysates using this bead with very high purity. Also, the mild non-denaturing elution condition preserves protein antigenicity.
TECHNICAL SPECIFICATIONS:
BEAD GEOMETRY & SIZE:    Spherical, ∼ 50 – 150 μm diameter
CROSS-LINKED:                     Yes
BEAD AGAROSE %:               4%
ACTIVATING GROUP:           Epoxy
SPACER ARM:                       12 atom spacer arm (10 carbons)
PURIFICATION CAPACITY:  ∼30 mg GST tagged fusion protein/ml pack bead
MATRIX STABILITY:             Stable in all commonly used reagents
STORAGE SOLUTION:          20% aqueous ethanol
STORAGE TEMPERATURE:   4°C to 8°C. DO NOT FREEZE.