Proteins of interest are generally tagged with His peptide for both an easy detection and simple purification by Ni2+-affinity chromatography. Anti-His antibody is the tool for detection of His-tag proteins by different immunological techniques.
This antibody is produced in rabbit by repeated injections of Immunogen.
Purification: Purified against peptide affinity chromatography.
Recommended Dilutions:
Western Blotting-1:2000; ELISA-1:10,000
Storage buffer: Tris Buffer pH 7.4, containing 1% BSA & 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).
*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubations and washings are in 1 X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.
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