Anti GAPDH Antibody

GAPDH is an enzyme of ~37kDa that catalyzes the conversion of glyceraldehyde 3-phosphate in the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In addition to this long-established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, and ER to Golgi vesicle shuttling. Anti-GAPDH antibody is primarily used to test purity of cytoplasmic cell fractionation and loading control. This antibody is produced in rabbit by repeated injections of Immunogen.
Purification: Purified against GAPDH protein (residues 13-335) affinity chromatography.
Recommended Dilutions:
Western Blotting-1:2000; ELISA-1:10,000
Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).
*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubations and washings are in 1X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.